关键词: 独立通风笼具, 洗消装置, 臭氧

Abstract: Objective　To improve the efficiency and safety of individually ventilated cage (IVC) for laboratory animals.Methods　This study developed a device for cleaning and disinfecting the IVC. The cleaning unit removes dust from the pipes through high-speed rotation of brushes and a negative pressure suction device. The disinfection unit uses high-concentration ozone as the disinfectant, achieving sterilization and decontamination via a closed internal circulation mode, thereby enhancing the efficiency of IVC cleaning and disinfection. The effectiveness of the device was verified by testing cleaning, disinfection, ventilation, ozone concentration, and air cleanliness. Bacterial test strips were placed in different parts of the cage rack to assess bacterial kill efficacy. Results　After cleaning, dust was removed from the interior and exterior of main pipes, vertical pipes, and air nozzles. All disinfection indicator cards reached the endpoint color, confirming the disinfection effect. Bacterial test strips in the experimental group yielded negative result, while those in the control group showed positive result, indicating a bacterial kill log value >3 (kill rate >99.9%). Post-cleaning, the air velocity at the cage rack outlets and air exchange rate of cage boxes were significantly higher than before cleaning (P< 0.001). After 5 min of operation, the ozone concentration inside the disinfection hood exceeded 2 140 mg/m3, while the indoor ozone concentration was 0, the air cleanliness reached Class 7. Conclusion　This device can efficiently and conveniently clean and disinfect IVC for laboratory animals, with clear cleaning and disinfection effects.

Key words: individually ventilated cage, cleaning and disinfection device, ozone